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Clearly these are exceptional individuals who have passion for their causes. The online tools allowed them to share their passion in numbers and geographies that otherwise would have been unthinkable with paper pledge forms. When we studied online campaigns we found that the aggregate data told a striking story: on average, the top 10 percent of online fundraisers accounted for more than 50 percent of all online dollars collected. When we shared these statistics with experienced offline fundraisers, the reaction we received was Of course we ve known for a long time that it only takes a small percentage of people to account for most of our fundraising. But this message hasn t played a central role in online fundraising discussions. Too often, we find organizations with no plan, or insufficient plans, to follow up and communicate with this digital donor elite in a way that could be distinguished from plans for their general online donors. Think of how your organization would steward someone who wrote a check for $25,000. How about someone who e-mailed friends, family, and colleagues to collectively raise $25,000 online

Adamson, N.J. and Reynolds, E.C. (1997) Rules relating electrophoretic mobility, charge and molecular size. Journal of Chromatography B, 699, 133 48. General review of theory of electrophoresis. Cruz, A., Marsh, D. and P rez-Gil, J. (1998) Rotational dynamics e of spin-labelled surfactant-associated proteins SP-B and SP-C in dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol bilayers. Biochemica et Biophysica Acta, 1415, 125 34. Issaq, H.J., Conrads, T.P., Janini, G.M. and Veenstra, T.M. (2002) Methods for fractionation, separation and pro ling of proteins and peptides. Electrophoresis, 23, 3048 61. A good general review of electrophoresis methods. Rathore, A.S. (2002) Theory of electroosmotic ow, retention and separation ef ciency in capillary electrochromatography. Electrophoresis, 23, 3827 46. A review of the theory of behaviour of buffers, charged and uncharged particles in open channels and porous media. Ji, L.T. and Choi, J.K. (2004) Usefulness of visible dyes for the staining of protein or DNA in electrophoresis. Electrophoresis, 25, 2429 39. Review of visible stains. Landers, J.P. (ed.) (2008) Handbook of Capillary and Microchip Electrophoresis and Related Techniques, 3rd edn, CRC Press, Boca Raton, FL, USA. Describes a broad range of applications of CE and related techniques. Westermeier, R. (2006) Electrophoresis in Practice A Guide to Methods and Applications of DNA and Protein Separations,

ormally, when a user clicks a link within your site, he or she is taken to an entirely new page. That new page loads into the browser, drawing the new page in its entirety. This means that elements, such as buttons, that appear on every page of your site, are redrawn on each page. (If they are graphics and are cached to the user s hard drive, they don t have to download again, but they still reload into the new page.) The alternative to this behavior is to present the user with multiple pages so that one page remains loaded and only the changing content has to load. This set-up is based on frames, which are the focus of this chapter. Frames enable you to display a group of completely independent, separate Web pages, all within one browser window at the same time. Each frame can have its own colors, backgrounds, images, links, and more. Frames can either be separated from other frames by thin borders, or be frameless, which means the border lines are hidden. Each frame can have a scrollbar to enable users to scroll through the frame s contents, or it can appear without a scrollbar, giving a more frameless look to the page. While frames provide some excellent convenience, they also present some downsides that must be considered. One downside is that the content page can t reliably be bookmarked; the menu command correctly bookmarks only the URL to the main (frameset) page. If the user creates a bookmark using a contextual menu, the bookmark captures the page within the frame,

I once knew a developer who was working on a classi ed project. He had clearance to see the source code but not the data, so every week his customer brought him a giant printout of the latest run with all of the data carefully clipped out with scissors. He would try to guess what was going on and make some suggestions so the customer s developers could try to x the code. Then the cycle repeated the next week. What an odd way to work! In other projects, the steps may work best in a different order. You may nd it better to brainstorm before visiting the customers site and watching them work. These are just steps that I ve found most useful in trying to understand the customers situation. You ll have to adjust them as necessary to t each of your projects. In this chapter, you learn how to: Understand the customers needs and motivations. Gather and document user requirements. Cull requirements from existing practices and information. Build use cases to understand the user s needs and to measure success or failure. Anticipate changes and future needs to build the most exible database possible.